Description
PPM1F, human recombinant is available at Gentaur for Next week delivery.
A negative regulator of cell stress response pathways.
Biomolecule/Target: PPM1F
Alternates names: Protein phosphatase 1F, CAMKP, CaMKPase, FEM-2, hFEM-2, POPX2
Synonyms: Protein phosphatase 1F, CAMKP, CaMKPase, FEM-2, hFEM-2, POPX2
Background Information: PPM1F is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. Recombinant human PPM1F protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Reconstitution Instructions: N/A
NCBI Gene Symbol: PPM1F
Gene ID: 9647
NCBI Accession: P49593
Additional Information
Size: |
100 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
E. coli. |
Purity by SDS-PAGE: |
≥90% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
N/A |
Activity (Specifications/test method): |
N/A |
Biological activity: |
Specific activity is > 1200 unit/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37ºC, pH 7.5 using 10 mM of substrate. |
Results: |
Specific activity is > 1200 unit/mg |
Molecular Weight: |
52 kDa (477 aa, 1-454 aa + His Tag) |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
1 mg/ml |
Appearance: |
Liquid |
Handling: |
Centrifuge the vial prior to opening. |