PPARγ (LBD) Human Recombinant (His-tagged)

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SKU:
7878-500
€185
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Description

PPARγ (LBD) Human Recombinant (His-tagged) is available at Gentaur for Next week delivery.

Recombinant Human PPARγ (LBD) can be used in the ligand screening assays, selectivity profiling, western blotting, ELISA, and numerous such applications

Biomolecule/Target: PPARγ

Alternates names: PPAR-gamma, Peroxisome Proliferator-activated Receptor γ Ligand Binding Domain, PPAR-gamma (LBD), NR1C3, PPARG (LBD)

Synonyms: PPAR-gamma, Peroxisome Proliferator-activated Receptor γ Ligand Binding Domain, PPAR-gamma (LBD), NR1C3, PPARG (LBD)

Background Information: The peroxisome proliferator activated receptor (PPAR) family of ligand-activated transcription factors consists of three subtypes encoded by separate genes: PPARα, PPARδ and PPARγ. Of these, PPARγ plays an important role in the regulation of fatty acid storage and glucose metabolism. The genes activated by PPARγ stimulate lipid uptake and adipogenesis by fat cells. Many naturally occurring molecules such as, polyunsaturated fatty acids like arachidonic acid and arachidonic acid metabolites, are known to bind and activate PPARγ. The binding of activating ligands to the ligand binding domain (LBD) of PPARγ promotes its heterodimerization with retinoic acid-like receptor (RXR), which results in the regulated expression of target genes involved in lipid metabolism. BioVision’s His-PPARγ (LBD) is a non-glycosylated protein expressed and purified from E. coli. It has been tested for functionality by its binding and subsequent displacement of 1,8-ANS assay.

Reconstitution Instructions: It can be further diluted to 0.5 mg/ml with 50 mM Tris, 100 mM NaCl, 1 mM TCEP, pH 8.0 containing 20% glycerol and stored at –80°C in aliquots. Avoid repeated freezing and thawing cycles.

NCBI Gene Symbol: PPARG

Gene ID: 5468

NCBI Accession:

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Additional Information

Size:
500 μg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
Human
Recombinant:
Yes
Source:
E. coli
Purity by SDS-PAGE:
≥90%
Assay:
SEC analysis
Purity:
N/A
Assay 2:
N/A
Endotoxin Level:
N/A
Activity (Specifications/test method):
N/A
Biological activity:
N/A
Results:
N/A
Molecular Weight:
34.8 kDa (NP_619726.2, 193–477 aa + N-terminal His6- tag)
Storage Temperature:
-80°C
Shelf Life:
6 months
Concentration:
2mg/ml
Appearance:
Liquid. In 50 mM Tris, 100 mM NaCl, 1 mM TCEP, pH 8.0 containing 20% glycerol
Handling:
Centrifuge the vial prior to opening.
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