Description
PPA1, human recombinant is available at Gentaur for Next week delivery.
Catalyzes the conversion of one molecule of pyrophosphate to two phosphate ions
Biomolecule/Target: PPA1
Alternates names: Inorganic pyrophosphatase, IOPPP, PP, PP1, SID6-8061
Synonyms: Inorganic pyrophosphatase, IOPPP, PP, PP1, SID6-8061
Background Information: PPA1 (Pyrophosphatase) belongs to the PPase family. This protein is an enzyme that catalyzes the conversion of one molecule of pyrophosphate to two phosphate ions. The hydrolysis of inorganic pyrophosphate (PPi) to two phosphate ions is utilized in many biochemical pathways to render reactions effectively irreversible. Inorganic pyrophosphatase catalyzes this hydrolysis reaction in the early steps of lipid degradation, a prominent example of this phenomenon. By promoting the rapid hydrolysis of pyrophosphate (PPi), Inorganic pyrophosphatase provides the driving force for the activation of fatty acids destined for oxidation. Recombinant human PPA1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Reconstitution Instructions: N/A
NCBI Gene Symbol: PPA1
Gene ID: 5464
NCBI Accession: Q15181
Additional Information
Size: |
100 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
E. coli. |
Purity by SDS-PAGE: |
≥95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
N/A |
Activity (Specifications/test method): |
N/A |
Biological activity: |
Specific activity is >28,000 pmoles/min/µg, and measured by its ability to hydrolyze pyrophosphate per minute at pH 9.0 at 25ºC |
Results: |
Specific activity is >28,000 pmoles/min/µg |
Molecular Weight: |
35.2 kDa (313 aa, 1-289 aa + His Tag) |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
0.5 mg/ml |
Appearance: |
Liquid |
Handling: |
Centrifuge the vial prior to opening. |