BioVision Proteins
Phosphoenolpyruvate Carboxykinase (PCK1), Human Recombinant
- SKU:
- P1229-50
Description
Phosphoenolpyruvate Carboxykinase (PCK1), Human Recombinant is available at Gentaur for Next week delivery.
Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP)
Biomolecule/Target: PCK1
Alternates names: PEPCK, PEPCK-C
Synonyms: PEPCK, PEPCK-C
Background Information: Phosphoenolpyruvate Carboxykinase (PCK1) belongs to the lyase family. In the presence of GTP, it catalyzes the reversible conversion of oxaloacetate (OAA) into phosphoenolpyruvate (PEP), GDP and CO2. In humans, two isoforms of PEPCK are found: cytosolic form (PCK1, also called PEPCK-C) and mitochondrial form (PEPCK-M). PCK1 is a control point enzyme which catalyzes a irreversible step in gluconeogenesis.
Reconstitution Instructions: Reconstitute to 2 mg/mL in 30% glycerol
NCBI Gene Symbol: PCK1
Gene ID: 5105
NCBI Accession: P35558
Additional Information
Size: |
50 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
E.coli |
Purity by SDS-PAGE: |
≥ 95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
N/A |
Activity (Specifications/test method): |
Measured by its ability to transfers a phosphate group from PEP to GDP and generates 1.0 µmol of pyruvate per minute at pH 7.5 at 37°C |
Biological activity: |
Specific activity >100 mU/mg |
Results: |
>100 mU/mg |
Molecular Weight: |
71.3 kDa |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Lyophilized |
Handling: |
Centrifuge the vial prior to opening. |