Phosphoenolpyruvate Carboxykinase (PCK1), Human Recombinant

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SKU:
P1229-50
€185
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Description

Phosphoenolpyruvate Carboxykinase (PCK1), Human Recombinant is available at Gentaur for Next week delivery.

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP)

Biomolecule/Target: PCK1

Alternates names: PEPCK, PEPCK-C

Synonyms: PEPCK, PEPCK-C

Background Information: Phosphoenolpyruvate Carboxykinase (PCK1) belongs to the lyase family. In the presence of GTP, it catalyzes the reversible conversion of oxaloacetate (OAA) into phosphoenolpyruvate (PEP), GDP and CO2. In humans, two isoforms of PEPCK are found: cytosolic form (PCK1, also called PEPCK-C) and mitochondrial form (PEPCK-M). PCK1 is a control point enzyme which catalyzes a irreversible step in gluconeogenesis.

Reconstitution Instructions: Reconstitute to 2 mg/mL in 30% glycerol

NCBI Gene Symbol: PCK1

Gene ID: 5105

NCBI Accession: P35558

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Additional Information

Size:
50 μg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
Human
Recombinant:
Yes
Source:
E.coli
Purity by SDS-PAGE:
≥ 95%
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
N/A
Endotoxin Level:
N/A
Activity (Specifications/test method):
Measured by its ability to transfers a phosphate group from PEP to GDP and generates 1.0 µmol of pyruvate per minute at pH 7.5 at 37°C
Biological activity:
Specific activity >100 mU/mg
Results:
>100 mU/mg
Molecular Weight:
71.3 kDa
Storage Temperature:
-20°C
Shelf Life:
12 months
Concentration:
N/A
Appearance:
Lyophilized
Handling:
Centrifuge the vial prior to opening.
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