Description
NME4, human recombinant is available at Gentaur for Next week delivery.
A ubiquitous enzyme that catalyze transfer of gamma-phosphates via a phosphohistidine intermediate.
Biomolecule/Target: NME4
Alternates names: Nucleoside diphosphate kinase, mitochondrial, NDK, NDPKD, nm23-H4, NM23D
Synonyms: Nucleoside diphosphate kinase, mitochondrial, NDK, NDPKD, nm23-H4, NM23D
Background Information: NME4, also known as nucleoside diphosphate kinase, mitochondrial, belongs to the NDK family. NME4 are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates. The enzymes are products of the nm23 gene family, which includes NME4. NME4 plays a major role in the synthesis of nucleoside triphosphates other than ATP. Recombinant human NME4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Reconstitution Instructions: N/A
NCBI Gene Symbol: NME4
Gene ID: 4833
NCBI Accession: O00746
Additional Information
Size: |
100 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
E. coli. |
Purity by SDS-PAGE: |
≥90% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
N/A |
Activity (Specifications/test method): |
N/A |
Biological activity: |
Specific activity is > 15 units/ml, in which one unit will convert 1.0 µmole each of TDP and ATP to TTP and ADP per minute at pH 7.6 at 25ºC in a coupled system with PK/LDH. |
Results: |
Specific activity is > 15 units/ml |
Molecular Weight: |
19.6 kDa (176 aa, 33-187 aa + His Tag) |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
0.5 mg/ml |
Appearance: |
Liquid |
Handling: |
Centrifuge the vial prior to opening. |
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