NFNB, bacterial recombinant

(No reviews yet) Write a Review
SKU:
7830-100
€185
Frequently bought together:

Description

NFNB, bacterial recombinant is available at Gentaur for Next week delivery.

An enzyme for activating prodrugs in antibody directed enzyme prodrug therapy

Biomolecule/Target: NFNB

Alternates names: Dihydropteridine reductase, NAD(P)H-dependent, oxygen-insensitive, dprA, nfsB, nfsI, ntr.

Synonyms: Dihydropteridine reductase, NAD(P)H-dependent, oxygen-insensitive, dprA, nfsB, nfsI, ntr.

Background Information: NFNB, also known as NFSB, shows the ability to reduce quinines. This protein is an enzyme for activating prodrugs in antibody directed enzyme prodrug therapy. It also capable of reducing nitrofurazone, quinones and the anti-tumor agent CB1954 (5-(aziridin-1-yl)-2, 4-dinitrobenzamide). The reduction of CB1954 results in the generation of cytotoxic species. Recombinant E.coli NFNB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Reconstitution Instructions: N/A

NCBI Gene Symbol: NFNB

Gene ID: 4493867

NCBI Accession: A1A8L0

View AllClose

Additional Information

Size:
100 μg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
E. Coli
Recombinant:
Yes
Source:
E. coli.
Purity by SDS-PAGE:
≥95%
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
N/A
Endotoxin Level:
N/A
Activity (Specifications/test method):
N/A
Biological activity:
Specific activity: > 2 units/ml One unit will oxidize 1.0 µmole of b-NADH to b-NAD with 6.7-Dimethyldihydropterine (quinonoid isomer) as the non-nucleotide substrate per minute at pH 7.2 at 25ºC
Results:
Specific activity: > 2 units/ml
Molecular Weight:
26.0 kDa (237 aa, 1-217 aa + His Tag)
Storage Temperature:
-20°C
Shelf Life:
12 months
Concentration:
1 mg/ml
Appearance:
Liquid
Handling:
Centrifuge the vial prior to opening.
View AllClose

0 Reviews

View AllClose