BioVision Proteins
Mutant Alanine Racemase Y354N, Active, Recombinant
- SKU:
- P1116-500
Description
Mutant Alanine Racemase Y354N, Active, Recombinant is available at Gentaur for Next week delivery.
Catalyzes the interconversion of L-alanine and D-alanine. Also weakly active on serine.
Biomolecule/Target: alr
Alternates names: Alanine Racemase Y354N, alr, dal
Synonyms: Alanine Racemase Y354N, alr, dal
Background Information: Mutant Alanine Racemase (Y354N) (mAR-Y354N), a pyridoxal 5-phosphate (PLP) dependent enzyme catalyzes the interconversion of the L-Serine to D-Serine. In WT Alanine Racemase Tyr354 plays a crucial role in defining the strict specificity of AR for alanine, in converting L-Alanine to D-Alanine, which is an important component of the peptidoglycan layer of bacterial cell wall. By mutating the active site Tyr 354 to Asn, the specificity of the enzyme changes and it becomes a racemase with dual specificity for L- Alanine and L-Serine.
Reconstitution Instructions: Reconstitute enzyme in 50 mM phosphate buffer, pH 7.4.
NCBI Gene Symbol: alr
Gene ID: 2506170
NCBI Accession: P10724
Additional Information
Size: |
500 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Geobacillus stearothermophilus, |
Recombinant: |
Yes |
Source: |
E. Coli |
Purity by SDS-PAGE: |
> 99% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
N/A |
Activity (Specifications/test method): |
Measured by its ability to convert 1 µmole of L-serine into D-serine per minute at 37 ºC and pH 7.4 in the presence of pyridoxal phosphate |
Biological activity: |
Specific activity is ≥ 50 mU/mg in which one unit enzyme converts 1 µmole of L-serine into D-serine per minute at 37 ºC and pH 7.4 in the presence of pyridoxal phosphate |
Results: |
≥ 50 mU/mg |
Molecular Weight: |
43 kDa |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Lyophilized |
Handling: |
Centrifuge the vial prior to opening. |
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