Description
IDO1, human recombinant is available at Gentaur for Next week delivery.
Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway
Biomolecule/Target: IDO1
Alternates names: Indoleamine 2,3-dioxygenase 1, IDO, IDO-1, INDO
Synonyms: Indoleamine 2,3-dioxygenase 1, IDO, IDO-1, INDO
Background Information: IDO1 is an indoleamine 2,3-dioxygenase - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, and serotonin. It is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its pericellular catabolization of the essential amino acid tryptophan. Recombinant human IDO1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Reconstitution Instructions: N/A
NCBI Gene Symbol: IDO1
Gene ID: 3620
NCBI Accession: P14902
Additional Information
Size: |
50 µg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
E. Coli |
Purity by SDS-PAGE: |
≥90% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
N/A |
Activity (Specifications/test method): |
Measured by the hydrolysis of 1.0 pmole of L-Tryptophan to N-formyl-Lkynurenine per minute at pH 6.5 at 25C. |
Biological activity: |
Specific activity is > 300 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of L-Tryptophan to N-formyl-Lkynurenine per minute at pH 6.5 at 25C. |
Results: |
>300 pmol/min/ug |
Molecular Weight: |
47.7 kDa |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
0.5 mg/ml |
Appearance: |
Liquid |
Handling: |
Centrifuge the vial prior to opening. |