BioVision Products
Human CellExp™ PCSK9, human recombinant
- SKU:
- 7265-20
Description
Human CellExp™ PCSK9, human recombinant is available at Gentaur for Next week delivery.
Plays a major regulatory role in cholesterol homeostasis
Biomolecule/Target: PCSK9
Alternates names: PCSK9, FH3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9, Proprotein convertase subtilisin/kexin type 9
Synonyms: PCSK9, FH3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9, Proprotein convertase subtilisin/kexin type 9
Background Information: Proprotein convertase subtilisin/kexin type 9 (PCSK9), is an enzyme which in humans is encoded by the PCSK9 gene. This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 100 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
NCBI Gene Symbol: PCSK9
Gene ID: 255738
NCBI Accession: Q8NBP7
Additional Information
Size: |
20 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
HEK 293 cells |
Purity by SDS-PAGE: |
≥97% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
< 0.1 ng/μg of protein (<1EU/μg) by LAL method |
Activity (Specifications/test method): |
Measured by its ability to bind with human LDLR in a functional ELISA Immobilized recombinant human PCSK9 at 10 μg/ml (100 μl/well) can bind biotinylated human LDLR. The ED50 for this effect is typically 15 ng/ml. |
Biological activity: |
Measured by its ability to bind with human LDLR in a functional ELISA Immobilized recombinant human PCSK9 at 10 μg/ml (100 μl/well) can bind biotinylated human LDLR. The ED50 for this effect is typically 15 ng/ml. |
Results: |
N/A |
Molecular Weight: |
This protein fused with polyhistidine tag at the C-terminus, has a calculated MW of 75.1 kDa. The predicted N-terminus is Gln 31. DTT-reduced Protein migrates as 20 kDa and 62 kDa due to glycosylation and proteolytic digestion. |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Lyophilized powder |
Handling: |
Centrifuge the vial prior to opening. |
Related Products
Human CellExp™ PCSK9, murine recombinant
BioVision Products
Human CellExp™ GAD1, human recombinant
BioVision Products
Human CellExp™ LDLR, human recombinant
BioVision Products
Human CellExp™ DKK3, human recombinant
BioVision Products
Human CellExp™ CD36, human recombinant
BioVision Products