Human CellExp™ MMP-1, human recombinant

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SKU:
7244-50
£135.37
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Description

Human CellExp™ MMP-1, human recombinant is available at Gentaur for Next week delivery.

Involved in the breakdown of extracellular matrix

Biomolecule/Target: MMP-1

Alternates names: MMP1, CLG, CLGN

Synonyms: MMP1, CLG, CLGN

Background Information: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive pro-proteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Thrombospondins, intervertebral disc proteins, regulate the effective levels of matrix metalloproteinases (MMPs) 2 and 9, which are key effectors of ECM remodeling. Matrix metalloproteinase-1 (MMP-1) also known as interstitial collagenase and fibroblast collagenase. MMP1 is expressed by fibroblasts, keratinocytes, endothelial cells, monocytes and macrophages. MMP-1 breaks down the interstitial collagens, types I, II, and III. MMP1 can degrade a broad range of substrates including types I, II, III, VII, VIII, and X collagens as well as casein, gelatin, myelin basic protein, L Selectin, proTNF, IL1β, IGFBP3, IGFBP5, pro MMP2 and pro MMP9.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

NCBI Gene Symbol: MMP-1

Gene ID: 4312

NCBI Accession: P03956

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Additional Information

Size:
50 μg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
Human
Recombinant:
Yes
Source:
HEK 293 cells
Purity by SDS-PAGE:
≥95%
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
HPLC
Endotoxin Level:
< 0.1 ng/μg of protein (<1EU/μg).
Activity (Specifications/test method):
Measured by its ability to cleave a fluorogenic peptide substrate Mca - KPLGL - Dpa - AR - NH2. The specific activity is > 200 pmol/min/µg, as measured under the described conditions.
Biological activity:
Measured by its ability to cleave a fluorogenic peptide substrate Mca - KPLGL - Dpa - AR - NH2. The specific activity is > 200 pmol/min/µg, as measured under the described conditions.
Results:
N/A
Molecular Weight:
This protein is fused with 6×His tag at the N-terminus, has a calculated MW of 52.7 kDa. The predicted N-terminus is Phe 20. DTT-reduced Protein migrates as 52-55 kDa.
Storage Temperature:
-20°C
Shelf Life:
12 months
Concentration:
N/A
Appearance:
Lyophilized powder
Handling:
Centrifuge the vial prior to opening.
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