BioVision Products
Human CellExp™ Fetuin A/ASHG, Human Recombinant
- SKU:
- 7142-50
Description
Human CellExp™ Fetuin A/ASHG, Human Recombinant is available at Gentaur for Next week delivery.
Exerts various calcium dependent physiological activities
Biomolecule/Target: Fetuin A
Alternates names: Alpha-2-HS-glycoprotein, Alpha-2-Z-globulin, Ba-alpha-2-glycoprotein
Synonyms: Alpha-2-HS-glycoprotein, Alpha-2-Z-globulin, Ba-alpha-2-glycoprotein
Background Information: Fetuin A/AHSG is a human plasma glycoprotein belonging to the Cystatin family of proteases inhibitors. It is highly expressed in embryonic cells and adult hepatocytes, and is expressed to a lesser extent in monocytes/macrophages. Fetuin A/AHSG is a major serum protein component that exerts various calcium dependent physiological activities, and can mediate growth signaling in certain tumor cells. It also can act as a natural antagonist against specific TGF-β and BMP signaling proteins. Recombinant human Fetuin-A is a heterodimeric protein containing a 282 amino acid A chain and a 27 amino acid B chain. Due to glycosylation, recombinant Fetuin-A migrates at an apparent molecular weight of 45 – 55 kDa by SDS-PAGE run under non-reducing conditions.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
NCBI Gene Symbol: FETUA
Gene ID: 197
NCBI Accession: P02765
Additional Information
Size: |
50 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
HEK 293 cells |
Purity by SDS-PAGE: |
≥95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
HPLC |
Endotoxin Level: |
< 0.1 ng/μg of protein (<1EU/μg). |
Activity (Specifications/test method): |
Determined by its ability to inhibit Cathepsin V cleavage of a fluorogenic peptide substrate ZLR-AMC (RLUs). The expected IC50 is ≤ 100nM. |
Biological activity: |
Determined by its ability to inhibit Cathepsin V cleavage of a fluorogenic peptide substrate ZLR-AMC (RLUs). The expected IC50 is ≤ 100nM. |
Results: |
N/A |
Molecular Weight: |
45-55 kDa |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Lyophilized powder |
Handling: |
Centrifuge the vial prior to opening. |