BioVision Products
Human CellExp™ EGFR/ErbB1, human recombinant
- SKU:
- 7396-10
Description
Human CellExp™ EGFR/ErbB1, human recombinant is available at Gentaur for Next week delivery.
A cell-surface receptor for members of the EGF-family of extracellular protein ligands.
Biomolecule/Target: ErbB1
Alternates names: EGFR, ERBB, ERBB1, HER1, PIG61, mENA
Synonyms: EGFR, ERBB, ERBB1, HER1, PIG61, mENA
Background Information: The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
NCBI Gene Symbol: EGFR
Gene ID: 1956
NCBI Accession: P00533
Additional Information
Size: |
10 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
HEK293 cells |
Purity by SDS-PAGE: |
≥95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
<1 EU/μg by LAL method |
Activity (Specifications/test method): |
N/A |
Biological activity: |
Measured by its binding ability in a functional ELISA. Immobilized human EGF at 10 μg/ml can bind human EGFR with a linear range of 0.5–750 ng/ml. |
Results: |
Measured by its binding ability in a functional ELISA. Immobilized human EGF at 10 μg/ml can bind human EGFR with a linear range of 0.5–750 ng/ml. |
Molecular Weight: |
This protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 69.5 kDa. The predicted N-terminus is Leu 25. DTT-reduced Protein migrates as 110-115 kDa due to glycosylation. |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Lyophilized |
Handling: |
Centrifuge the vial prior to opening. |