BioVision Proteins
Human CellExp™CEACAM6/CD66c, human recombinant
- SKU:
- 7837-50
Description
Human CellExp™CEACAM6/CD66c, human recombinant is available at Gentaur for Next week delivery.
Affects the sensitivity of tumor cells to adenovirus infection. Widely used as a tumor marker in serum immunoassay determinations of carcinomas.
Biomolecule/Target: CEACAM6/CD66c
Alternates names: CEACAM6, CD66c, CEAL, NCA
Synonyms: CEACAM6, CD66c, CEAL, NCA
Background Information: Carcinoembryonic antigen-related cell adhesion molecule 6 (non-specific cross reacting antigen) (CEACAM6) also known as CD66c (Cluster of Differentiation 66c), CEAL, NCA, and is one of seven human CEACAM family members within the immunoglobulin superfamily. In humans, CEACAMs include type I transmembrane proteins (CEACAM1, CEACAM3, and CEACAM4) and GPI-linked molecules (CEACAM5 through CEACAM8). There is no human CEACAM2. CEACAM 6 contains one N-terminal V-type Ig-like domain (N domain), followed by two C2-type Ig-like domains. It shows considerable glycosylation, including (sialyl) LewisX, which mediates binding to E-selectin, galectins and some bacterial fimbrae. CEACAM-6 is expressed by granulocytes and their progenitors. It is also expressed by epithelia of various organs and is upregulated in pancreatic and colon adenocarcinomas, as well as hyperplastic polyps. Resistance to adhesion-related apoptosis in tumor cells is conferred in the condition of CEACAM6 overexpression.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.
NCBI Gene Symbol: CEACAM6
Gene ID: 4680
NCBI Accession: P40199
Additional Information
Size: |
50 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
HEK 293 cells |
Purity by SDS-PAGE: |
≥95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
< 1.0 EU per μg of the rhCEACAM6 by the LAL method. |
Activity (Specifications/test method): |
Measured by the ability of the immobilized protein to support the adhesion of calcium ionophore treated human neutrophils. When 2 x 105 cells/well are added to CEACAM6 coated plates (10 μg/mL, 100 μL/well), 45-70% of the cells will adhere after 20 mi |
Biological activity: |
Measured by the ability of the immobilized protein to support the adhesion of calcium ionophore treated human neutrophils. When 2 x 105 cells/well are added to CEACAM6 coated plates (10 μg/mL, 100 μL/well), 45-70% of the cells will adhere after 20 mi |
Results: |
Measured by the ability of the immobilized protein to support the adhesion of calcium ionophore treated human neutrophils. When 2 x 105 cells/well are added to CEACAM6 coated plates (10 μg/mL, 100 μL/well), 45-70% of the cells will adhere after 20 mi |
Molecular Weight: |
This protein rhCEACAM6 is fused with 6×his tag at C-terminus, has a calculated MW of 32 kDa expressed. The predicted N-terminus is Lys35. Protein migrates as 35-65 kDa in reduced SDS-PAGE resulting from glycosylation. |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Lyophilized |
Handling: |
Centrifuge the vial prior to opening. |