BioVision Products
Human CellExp™ CD47, human recombinant
- SKU:
- 7385-10
Description
Human CellExp™ CD47, human recombinant is available at Gentaur for Next week delivery.
Plays a role in cell adhesion modulation of integrins
Biomolecule/Target: CD47
Alternates names: CD47, MER6, IAP, OA3
Synonyms: CD47, MER6, IAP, OA3
Background Information: Leukocyte surface antigen CD47 also known as antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances super antigen-dependent T-cell-mediated proliferation and co-stimulates T-cell activation.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
NCBI Gene Symbol: CD47
Gene ID: 961
NCBI Accession: Q08722
Additional Information
Size: |
10 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
HEK293 cells |
Purity by SDS-PAGE: |
≥95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
<1 EU/μg by LAL method |
Activity (Specifications/test method): |
N/A |
Biological activity: |
Measured by its binding ability in a functional ELISA. When Recombinant Human CD172a / SIRP alpha Fc Chimera is coated at 1 µg/mL, rhCD47 binds with apparent Kd < 1 nM. |
Results: |
Measured by its binding ability in a functional ELISA. When Recombinant Human CD172a / SIRP alpha Fc Chimera is coated at 1 µg/mL, rhCD47 binds with apparent Kd < 1 nM. |
Molecular Weight: |
This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 14.5 kDa. The predicted N-terminus is Gln 19. DTT-reduced Protein migrates as 35-43 kDa due to glycosylation. |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Lyophilized |
Handling: |
Centrifuge the vial prior to opening. |