Human CellExp™ CD28, Human/ Cynomolgus / Rhesus macaque Recombinant

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SKU:
P1471-50
$719.07
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Description

Human CellExp™ CD28, Human/ Cynomolgus / Rhesus macaque Recombinant is available at Gentaur for Next week delivery.

T-cell-specific surface glycoprotein CD28

Biomolecule/Target:

Alternates names: T-cell-specific surface glycoprotein CD28, CD antigen: TP44, Tp44, T-Cell-Specific Surface Glycoprotein, CD28 Molecule

Synonyms: T-cell-specific surface glycoprotein CD28, CD antigen: TP44, Tp44, T-Cell-Specific Surface Glycoprotein, CD28 Molecule

Background Information: Human CD28 (Cluster of Differentiation 28) is expressed on the cell surface as a glycosylated, disulfide-linked homodimer. CD28 is the founding member of a subfamily of costimulatory molecules characterized by an extracellular variable immunoglobulin-like domain. Other members of the subfamily include ICOS, CTLA4, PD1, PD1H, and BTLA. CD28 and CTLA4 are highly homologous and compete for the same ligands B7-1 (CD80) and B7-2 (CD86). CD28 is expressed constitutively on almost all human CD4 T cells and approximately 50% of CD8 T cells. CD28 has diverse effects on T cell functions including the induction of cell proliferation and cytokine production and promotion of T-cell survival. Experiments showed that CD28 and CTLA4 have opposing effects on T cell stimulation. CD28 provides an activating signal and CTLA4 provides an inhibitory signal. Translation of a basic understanding of CD28 function into immunomodulatory therapeutics has been uneven, with both successes and failures from pre-clinical studies.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4.

NCBI Gene Symbol: CD28

Gene ID: 940

NCBI Accession: P10747

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Additional Information

Size:
50 µg
Adwords:
False
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
Human/ Cynomolgus / Rhesus macaque
Recombinant:
True
Source:
HEK 293 cells
Purity by SDS-PAGE:
>90%
Assay:
SDS-PAGE
Biological activity:
Human biotinylated CD28 can bind to immobilized human CD86 (BioVision Cat# 7496 at 5 μg/ml, 100 μl/well) with a linear detection range of 0.08 to 0.6 ug/ml.
Molecular Weight:
This protein is fused with a polyhistidine tag at the C-terminus with the calculated molecular weight of ~16 kDa. The predicted N-terminus is Asn 19 (UniProtKB – P10747 CD28_HUMAN). DTT-reduced protein shows a band about 35-45 kDa due to heavy glycos
Storage Temperature:
-20°C
Shelf Life:
12 months
Appearance:
Lyophilized powder
Handling:
Centrifuge the vial prior to opening.
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