Description
Homocysteine α,γ-Lyase (rHCYase), Active, Recombinant is available at Gentaur for Next week delivery.
Enzyme that can metabolize homocysteine into α-keto butyrate, hydrogen sulfide and ammonia.
Biomolecule/Target: mgl1
Alternates names: α,γ-Homocysteinase, Methionine gamma-lyase, mgl1
Synonyms: Homocysteine α,γ-Lyase (rHCYase)
Background Information: Recombinant Homocysteine α,γ-lyase is a pyridoxal-5’-phosphate dependent enzyme. It is a mutant of homocysteinase from Trichomonas vaginalis encoded by mgl1 gene, containing three point mutations, such as; Phe47Leu, Asp172Glu, Ser308Tyr. Its native molecular mass is 170 kD, with four seemingly identical subunits, each with molecular mass of 43-45 kD. The enzyme can metabolize homocysteine into α-keto butyrate, hydrogen sulfide and ammonia.
Reconstitution Instructions: Reconstitute in 40 mM Sodium phosphate buffer, pH 8.1.
NCBI Gene Symbol: mgl1
NCBI Accession: O15564
Additional Information
Size: |
200 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Trichomonas vaginalis |
Recombinant: |
Yes |
Source: |
E. Coli |
Purity by SDS-PAGE: |
> 95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
N/A |
Activity (Specifications/test method): |
Measured by its ability to convert 1 µmole of homocysteine into hydrogen sulfide, per minute at 25 ºC and pH 8.1 in the presence of pyridoxal phosphate |
Biological activity: |
Specific activity is ≥ 5 mU/mg in which one Unit enzyme converts 1 µmole of homocysteine into hydrogen sulfide, per minute at 25 ºC and pH 8.1 in the presence of pyridoxal phosphate |
Results: |
≥ 5 mU/mg |
Molecular Weight: |
43 kDa |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Lyophilized |
Handling: |
Centrifuge the vial prior to opening. |