Description
HAGH, human recombinant is available at Gentaur for Next week delivery.
A thiolesterase which hydrolyses S-lactoyl-glutathione to reduced glutathione and D-lactate.
Biomolecule/Target: HAGH
Alternates names: Hydroxyacylglutathione hydrolase, GLO, GLX2, Glyoxalase II, HAGH1
Synonyms: Hydroxyacylglutathione hydrolase, GLO, GLX2, Glyoxalase II, HAGH1
Background Information: HAGH is a member of the glyoxalase family and a thiolesterase which hydrolyses S-lactoyl-glutathione to reduced glutathione and D-lactate. This protein is a detoxifying enzyme of glycolysis byproduct methylglyoxal and a target of p63 and p73 and serves as a pro-survival factor of the p53 family. It exists only as a monomer and binds two zinc ions per subunit. Recombinant human HAGH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Reconstitution Instructions: N/A
NCBI Gene Symbol: HAGH
Gene ID: 3029
NCBI Accession: Q16775
Additional Information
Size: |
50 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
E. coli. |
Purity by SDS-PAGE: |
≥95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
N/A |
Activity (Specifications/test method): |
N/A |
Biological activity: |
Specific activity is > 1.8 unit/ml, in which one unit will hydrolyze 1.0 µmole of S-lactoylglutathione per minute at pH 7.5 at 25ºC. |
Results: |
Specific activity is > 1.8 unit/ml |
Molecular Weight: |
31.4 kDa (284 aa, 1-260 aa + His Tag) |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
0.5 mg/ml |
Appearance: |
Liquid |
Handling: |
Centrifuge the vial prior to opening. |