Description
GSK-3β, human recombinant is available at Gentaur for Next week delivery.
A phosphorylating and an inactivating agent of glycogen synthase
Biomolecule/Target:
Alternates names: GSK, Glycogen synthase kinase-3 (GSK-3)
Synonyms: GSK, Glycogen synthase kinase-3 (GSK-3)
Background Information: Glycogen synthase kinase-3 (GSK-3) is a serine/theronine kinase that was first characterized by its role in intermediary metabolism. In mammals, serine kinase is encoded by two related genes called GSK-3α (51 kDa) and GSK-3β (46 kDa). GSK-3 has been implicated in the regulation of the c-Jun/AP-1 transcription factor and other intracellular signaling pathways.
Reconstitution Instructions: Reconstitute to 1 mg/ml in PBS containing 15% glycerol. The solution should be stored at –70°C, stable for up to 3 months.
NCBI Gene Symbol: GSK3B
Gene ID: 2932
NCBI Accession: P49841
Additional Information
Size: |
1 mg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
E. coli. |
Purity by SDS-PAGE: |
≥90% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
HPLC |
Endotoxin Level: |
N/A |
Activity (Specifications/test method): |
Activity of GSK-3β was determined by an in vitro kinase assay using 100 ng Tau as substrate and 1 µg of the recombinant GSK-3β in a 20 µl reaction containing 25 mM Tris-HCl, pH 7.5, 5 mM β-glycerol phosphate, 12 mM MgCl₂, 2 mM DTT, 0.1 M Na3VO4, and |
Results: |
N/A |
Molecular Weight: |
53.0-56.0 kDa |
Storage Temperature: |
-70°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Lyophilized powder |
Handling: |
Centrifuge the vial prior to opening. |