Description
GDNF, rat recombinant is available at Gentaur for Next week delivery.
Promotes dopamine uptake and survival and morphological differentiation of midbrain neurons
Biomolecule/Target: GDNF
Alternates names: Glial-Derived Neurotrophic Factor, ATF-1
Synonyms: Glial-Derived Neurotrophic Factor, ATF-1
Background Information: GDNF is a disulfide-linked homodimeric neurotrophic factor structurally related to Artemin, Neurturin and Persephin. These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRα (α1-α4) receptors. GDNF specifically promotes dopamine uptake and survival and morphological differentiation of midbrain neurons. Using Parkinson’s disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability. The functional rat GDNF ligand is a disulfide linked homodimer, of two 15.0 kDa polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, one of which is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
NCBI Gene Symbol: GDNF
Gene ID: 25453
NCBI Accession: Q07731
Additional Information
Size: |
50 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Murine |
Recombinant: |
Yes |
Source: |
E. coli. |
Purity by SDS-PAGE: |
≥98% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
HPLC |
Endotoxin Level: |
< 0.1 ng/μg of protein (<1EU/μg). |
Activity (Specifications/test method): |
Determined by its ability to stimulate the proliferation of rat C6 cells. |
Biological activity: |
Determined by its ability to stimulate the proliferation of rat C6 cells. |
Results: |
N/A |
Molecular Weight: |
15 kDa |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Lyophilized powder |
Handling: |
Centrifuge the vial prior to opening. |