G6PD, Human Recombinant

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SKU:
7532-100
$719.07
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Description

G6PD, Human Recombinant is available at Gentaur for Next week delivery.

Rate-limiting enzyme of the pentose phosphate pathway

Biomolecule/Target: G6PD

Alternates names: Glucose-6-phosphate 1-dehydrogenase, Zwf.

Synonyms: Glucose-6-phosphate 1-dehydrogenase, Zwf.

Background Information: Glucose-6-phosphate dehydrogenase is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH. G6PD converts glucose-6-phosphate into 6-phosphoglucono-delta-lactone and simultaneously produce NADPH. The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. G6PD deficiency cause acute hemolytic anemia. Recombinant human G6PD protein, fused to His-tag at N-terminus, was expressed in Hi-5 cells using baculovirus expression system and purified by using conventional chromatography.

Reconstitution Instructions: N/A

NCBI Gene Symbol: G6PD

Gene ID: 2539

NCBI Accession: P11413

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Additional Information

Size:
100 μg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
Human
Recombinant:
Yes
Source:
Hi-5 cells, Baculovirus system
Purity by SDS-PAGE:
≥95%
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
N/A
Endotoxin Level:
N/A
Activity (Specifications/test method):
N/A
Biological activity:
Specific activity is > 7 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP. One unit oxidizes 1.0 µmole D-glucose-6-phosphate to 6-phospho-Dgluconate per min in the presence of be
Results:
Specific activity is > 7 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP. One unit oxidizes 1.0 µmole D-glucose-6-phosphate to 6-phospho-Dgluconate per min in the presence of be
Molecular Weight:
61.4 kDa (515 aa, 1-515 aa)
Storage Temperature:
-20°C
Shelf Life:
6 months
Concentration:
0.5 mg/ml
Appearance:
Liquid
Handling:
Centrifuge the vial prior to opening.
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