BioVision Proteins
E. coli Recombinant Carbonic anhydrase
- SKU:
- P1049-50
Description
E. coli Recombinant Carbonic anhydrase is available at Gentaur for Next week delivery.
Catalyzes the reversible hydration of carbon dioxide in various biological processes.
Biomolecule/Target: can
Alternates names: Carbonate dehydratase, CAN, yadF
Synonyms: Carbonate dehydratase, CAN, yadF
Background Information: Carbonic anhydrase (CA) is an enzyme that catalyses rapid conversion of carbon dioxide to bicarbonate and protons (CO2 + H2O ↔ HCO3− + H+). Most carbonic anhydrases contain a zinc ion in their active site and the primary function of this enzyme is known to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide of tissues. Carbonic anhydrases have been found in all kingdoms of life. Recombinant carbonic anhydrase fused to His-tag, was expressed in E.coli and purified by conventional chromatography techniques.
Reconstitution Instructions: N/A
NCBI Gene Symbol: CAN
Gene ID: 944832
NCBI Accession: P61517
Additional Information
Size: |
50 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
E. coli |
Recombinant: |
Yes |
Source: |
E. coli |
Purity by SDS-PAGE: |
> 95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
N/A |
Activity (Specifications/test method): |
Measured by its ability to hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C. |
Biological activity: |
Specific activity is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C. |
Results: |
>1,000 pmol/min/ug |
Molecular Weight: |
27 kDa |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Liquid |
Handling: |
Centrifuge the vial prior to opening. |