E. coli Recombinant Carbonic anhydrase

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SKU:
P1049-50
€185
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Description

E. coli Recombinant Carbonic anhydrase is available at Gentaur for Next week delivery.

Catalyzes the reversible hydration of carbon dioxide in various biological processes.

Biomolecule/Target: can

Alternates names: Carbonate dehydratase, CAN, yadF

Synonyms: Carbonate dehydratase, CAN, yadF

Background Information: Carbonic anhydrase (CA) is an enzyme that catalyses rapid conversion of carbon dioxide to bicarbonate and protons (CO2 + H2O ↔ HCO3− + H+). Most carbonic anhydrases contain a zinc ion in their active site and the primary function of this enzyme is known to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide of tissues. Carbonic anhydrases have been found in all kingdoms of life. Recombinant carbonic anhydrase fused to His-tag, was expressed in E.coli and purified by conventional chromatography techniques.

Reconstitution Instructions: N/A

NCBI Gene Symbol: CAN

Gene ID: 944832

NCBI Accession: P61517

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Additional Information

Size:
50 μg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
E. coli
Recombinant:
Yes
Source:
E. coli
Purity by SDS-PAGE:
> 95%
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
N/A
Endotoxin Level:
N/A
Activity (Specifications/test method):
Measured by its ability to hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.
Biological activity:
Specific activity is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.
Results:
>1,000 pmol/min/ug
Molecular Weight:
27 kDa
Storage Temperature:
-20°C
Shelf Life:
12 months
Concentration:
N/A
Appearance:
Liquid
Handling:
Centrifuge the vial prior to opening.
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