Description
DUSP21, human recombinant is available at Gentaur for Next week delivery.
Catalyzes dephosphorylation of phosphotyrosine and phosphothreonine residues.
Biomolecule/Target: DUSP21
Alternates names: Dual specificity protein phosphatase 21
Synonyms: Dual specificity protein phosphatase 21
Background Information: DuSP21 (Dual specificity protein phosphatase 21) belongs to the protein-tyrosine phosphatase family. Dual specificity protein phosphatases (DuSPs) inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. This enzyme can dephosphorylate single and diphosphorylated synthetic MAPK peptides, with preference for the phosphotyrosine and diphosphorylated forms over phosphothreonine. It is exclusively expressed in testis where is preferentially dephosphorylates phosphotyrosine residues in MAPK peptides. Recombinant human DuSP21 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Reconstitution Instructions: N/A
NCBI Gene Symbol: DUSP21
Gene ID: 63904
NCBI Accession: Q9H596
Additional Information
Size: |
50 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
E. coli. |
Purity by SDS-PAGE: |
≥90% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
N/A |
Activity (Specifications/test method): |
N/A |
Biological activity: |
Specific activity is > 1800 unit/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37ºC, pH7.5 using 10 mM of substrate. |
Results: |
Specific activity is > 1800 unit/mg |
Molecular Weight: |
24.1 kDa (214 aa, 1-190 aa + His Tag) |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
0.25 mg/ml |
Appearance: |
Liquid |
Handling: |
Centrifuge the vial prior to opening. |