Description
DUSP10, human recombinant is available at Gentaur for Next week delivery.
Negatively regulates members of the MAPK superfamily
Biomolecule/Target: DUSP10
Alternates names: Dual specificity protein phosphatase 10, MKP5
Synonyms: Dual specificity protein phosphatase 10, MKP5
Background Information: DuSP10 (Dual specificity protein phosphatase 10) belongs to the protein-tyrosine phosphatase family. Dual specificity protein phosphatases (DuSPs) inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the MAPK superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. DuSP10 has been shown to interact with MAPK14 and MAPK8. When expressed in mammalian cells, DuSP10 blocks the enzymatic activation of MAP kinases with the selectivity p38 approximately JNK/SAPK >> ERK. Recombinant human DuSP10 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Reconstitution Instructions: N/A
NCBI Gene Symbol: DUSP10
Gene ID: 11221
NCBI Accession: Q9Y6W6
Additional Information
Size: |
50 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Human |
Recombinant: |
Yes |
Source: |
E. coli. |
Purity by SDS-PAGE: |
≥90% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
N/A |
Activity (Specifications/test method): |
N/A |
Biological activity: |
Specific activity is > 1600 unit/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37ºC, pH 7.5 using 10 mM of substrate. |
Results: |
Specific activity is > 1600 unit/mg |
Molecular Weight: |
40.4 kDa (359 aa, 149-482 aa + His Tag) |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
0.25 mg/ml |
Appearance: |
Liquid |
Handling: |
Centrifuge the vial prior to opening. |