Description
D-Glucose Isomerase, Recombinant is available at Gentaur for Next week delivery.
Involved in D-xylose catabolism
Biomolecule/Target: D-Glucose Isomerase
Alternates names: D-Xylose Isomerase, fructose-isomerase, D-xylose aldose-ketose-isomerase, D-xylose ketoisomerase, D-xylose ketol-isomerase
Synonyms: D-Xylose Isomerase, fructose-isomerase, D-xylose aldose-ketose-isomerase, D-xylose ketoisomerase, D-xylose ketol-isomerase
Background Information: Glucose Isomerase catalyzes the reversible isomerization of glucose to fructose and that of xylose to xylulose. The enzyme is widely used in the industrial production of high-fructose corn syrup (HFCS) as well as in bioethanol fermentation from hemicellulosic hydrolysates.Recombinant Glucose Isomerase protein (S. rubiginosus), fused to His-tag at N-terminus, was expressed in E.coli and purified using propriety chromatography techniques.
Reconstitution Instructions: Spin at >300 x g for 10 minutes and remove the supernatant. Dissolve the pellets in PBS or appropriate buffer.
NCBI Gene Symbol: xylA
NCBI Accession: P24300
Additional Information
Size:  | 
                                                    500 mU  | 
                                                
Country of Manufacturing Origin:  | 
                                                    USA  | 
                                                
Country of Animal Origin:  | 
                                                    USA  | 
                                                
Gene Source:  | 
                                                    Streptomyces rubiginosus  | 
                                                
Recombinant:  | 
                                                    Yes  | 
                                                
Source:  | 
                                                    E.coli  | 
                                                
Purity by SDS-PAGE:  | 
                                                    ≥95%  | 
                                                
Assay:  | 
                                                    SDS-PAGE  | 
                                                
Purity:  | 
                                                    N/A  | 
                                                
Assay 2:  | 
                                                    N/A  | 
                                                
Endotoxin Level:  | 
                                                    N/A  | 
                                                
Activity (Specifications/test method):  | 
                                                    Measured by its ability to convert 1.0 µmol of Fructose to Glucose per min. at pH 8.0 at 37 °C.  | 
                                                
Biological activity:  | 
                                                    > 5 mU/mg. The activity of D-Glucose isomerase is determined by using the D-Glucose Isomerase Activity Assay Kit (Cat. No. K177).  | 
                                                
Results:  | 
                                                    > 5 mU/mg  | 
                                                
Molecular Weight:  | 
                                                    45.4 kDa  | 
                                                
Storage Temperature:  | 
                                                    -20°C  | 
                                                
Shelf Life:  | 
                                                    12 months  | 
                                                
Concentration:  | 
                                                    N/A  | 
                                                
Appearance:  | 
                                                    Ammonium sulfate suspension  | 
                                                
Handling:  | 
                                                    Centrifuge the vial prior to opening.  |