Description
D-Glucose Isomerase, Recombinant is available at Gentaur for Next week delivery.
Involved in D-xylose catabolism
Biomolecule/Target: D-Glucose Isomerase
Alternates names: D-Xylose Isomerase, fructose-isomerase, D-xylose aldose-ketose-isomerase, D-xylose ketoisomerase, D-xylose ketol-isomerase
Synonyms: D-Xylose Isomerase, fructose-isomerase, D-xylose aldose-ketose-isomerase, D-xylose ketoisomerase, D-xylose ketol-isomerase
Background Information: Glucose Isomerase catalyzes the reversible isomerization of glucose to fructose and that of xylose to xylulose. The enzyme is widely used in the industrial production of high-fructose corn syrup (HFCS) as well as in bioethanol fermentation from hemicellulosic hydrolysates.Recombinant Glucose Isomerase protein (S. rubiginosus), fused to His-tag at N-terminus, was expressed in E.coli and purified using propriety chromatography techniques.
Reconstitution Instructions: Spin at >300 x g for 10 minutes and remove the supernatant. Dissolve the pellets in PBS or appropriate buffer.
NCBI Gene Symbol: xylA
NCBI Accession: P24300
Additional Information
Size: |
500 mU |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Streptomyces rubiginosus |
Recombinant: |
Yes |
Source: |
E.coli |
Purity by SDS-PAGE: |
≥95% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
N/A |
Endotoxin Level: |
N/A |
Activity (Specifications/test method): |
Measured by its ability to convert 1.0 µmol of Fructose to Glucose per min. at pH 8.0 at 37 °C. |
Biological activity: |
> 5 mU/mg. The activity of D-Glucose isomerase is determined by using the D-Glucose Isomerase Activity Assay Kit (Cat. No. K177). |
Results: |
> 5 mU/mg |
Molecular Weight: |
45.4 kDa |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Ammonium sulfate suspension |
Handling: |
Centrifuge the vial prior to opening. |