Caspase-3, mouse recombinant

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SKU:
1183-25
£135.37
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Description

Caspase-3, mouse recombinant is available at Gentaur for Next week delivery.

A cysteine-aspartic acid protease involved in apoptosis

Biomolecule/Target: Caspase-3

Alternates names: Caspase-3, Short name=CASP-3, Apopain, Cysteine protease CPP32, Short name=CPP-32, FLICE, Protein Yama, SREBP cleavage activity 1, Short name=SCA-1

Synonyms: Caspase 3

Background Information: Caspase-3 (also know as CPP32, Yama and apopain) is a major member of the caspase-family of cysteine proteases. Caspase-3 exists in cells as an inactive 32 kDa proenzyme. During apoptosis procaspase-3 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases, such as caspase-6 (Mch2), caspase-8 (Flice) and grazyme B. The processed form of caspase-3 consists of large (17 kD) and small (11 kD) subunits which associate to form the active enzyme. The active caspase-3 has been shown involving in the proteolysis of several important molecules, such as poly (ADP-ribose) polymerase (PARP), the sterol regulatory element binding proteins (SREBPs), focal adhesion kinase (FAK), and others. The recombinant active human caspase-3 expressed in E. coli spontaneously undergoes autoprocessing to yield subunits characteristic of the native enzyme. The active caspase-3 preferentially cleaves caspase-3 substrates (e.g., DEVD-AFC or DEVD-pNA) and is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-DEVD-pNA (Cat. #1008-200) or Ac-DEVD-AFC (1007-200).

Reconstitution Instructions: Reconstitute to 1 unit per µl in PBS containing 15% glycerol

NCBI Gene Symbol: CASP3

Gene ID: 12367

NCBI Accession: Q8BNT4

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Additional Information

Size:
25 units
Adwords:
Yes
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
Mouse
Recombinant:
Yes
Source:
E. coli
Purity by SDS-PAGE:
N/A
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
HPLC
Endotoxin Level:
N/A
Activity (Specifications/test method):
300000 units/mg
Biological activity:
N/A
Results:
N/A
Molecular Weight:
of large (17 kD) and small (11 kD) subunits
Storage Temperature:
-70°C
Shelf Life:
12 months
Concentration:
N/A
Appearance:
Lyophilized powder
Handling:
Centrifuge the vial prior to opening.
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