Caspase-1, mouse recombinant

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SKU:
1181-25
$183
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Description

Caspase-1, mouse recombinant is available at Gentaur for Next week delivery.

A cysteine protease involved in apoptosis

Biomolecule/Target: Caspase-1

Alternates names: Caspase-1, Short name=CASP-1, Interleukin-1 beta convertase, Short name=IL-1BC, Interleukin-1 beta-converting enzyme, Short name=ICE, IL-1 beta-converting enzyme, p45

Synonyms: Caspase 1

Background Information: Caspase-1 (also know as ICE) is a prototypical member of the caspase-family of cysteine proteases. Caspase-1 exists in cells as an inactive 45 kDa proenzyme. The pro-enzyme is matured by proteolysis to yield large (20 kD) and small (10 kD) subunits. The active caspase-1 is a heterotetramer consisting of two large and two small subunits. To date the regulatory mechanism of caspase-1 activation and the role of caspase-1 in apoptosis are poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive proenzyme form. The recombinant active mouse caspase-1 was expressed in E. coli. The active caspase-1 preferentially cleaves caspase-1 substrates (e.g., YVAD-AFC or YVAD-pNA) and is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-YVAD-pNA (Cat. #1104-200) or Ac-YVAD-AFC (1103-200).

Reconstitution Instructions: Reconstitute to 1 unit per µl in PBS containing 15% glycerol.

NCBI Gene Symbol: CASP1

Gene ID: 12362

NCBI Accession: P29452

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Additional Information

Size:
25 units
Adwords:
Yes
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Gene Source:
Mouse
Recombinant:
Yes
Source:
E. coli
Purity by SDS-PAGE:
N/A
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
HPLC
Endotoxin Level:
N/A
Activity (Specifications/test method):
20000 units/mg
Biological activity:
N/A
Results:
N/A
Molecular Weight:
large (20 kD) and small (10 kD) subunits.
Storage Temperature:
-70°C
Shelf Life:
12 months
Concentration:
N/A
Appearance:
Semi-Dry
Handling:
Centrifuge the vial prior to opening.
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