Description
Akt2, Active is available at Gentaur for Next week delivery.
An important signaling molecule in the Insulin signaling pathway
Biomolecule/Target:
Alternates names: Akt, RAC-alpha serine/threonine-protein kinase
Synonyms: Akt, RAC-alpha serine/threonine-protein kinase
Background Information: Akt2 or Protein Kinase B β (PKBβ) is a serine/threonine kinase that is a member of the Akt family. Akt2 like the other Akt members is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, and cell motility (1). The PI3K generates phosphatidylinositol-3, 4, 5-trisphosphate (PIP3), a lipid second messenger essential for the translocation of Akt2 to the plasma membrane where it is phosphorylated and activated by phosphoinositide-dependent kinase-1 (PDK-1) (2) and phosphoinositide-dependent kinase-2. Akt1 has numerous cellular substrates including proteins, which promote the inhibition of apoptosis such as the Forkhead transcription factors and the Bcl-2 family member Bad (3).
Reconstitution Instructions: N/A
NCBI Gene Symbol: AKT2
Gene ID: 208
NCBI Accession: P31751
Additional Information
Size: |
5 μg |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Recombinant: |
Yes |
Source: |
Baculovirus (Sf9 insect cells) |
Purity by SDS-PAGE: |
≥90% |
Assay: |
SDS-PAGE |
Purity: |
N/A |
Assay 2: |
HPLC |
Endotoxin Level: |
N/A |
Activity (Specifications/test method): |
46 nmol phosphate incorporated into Akt/SGK substrate (RPRAATF) per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP and total of 0.83 µCi/µl P-32. |
Biological activity: |
N/A |
Results: |
N/A |
Molecular Weight: |
58.0 kDa |
Storage Temperature: |
-80°C |
Shelf Life: |
12 months |
Concentration: |
5 µg/50 µl |
Appearance: |
Liquid |