Akt1, Active

(No reviews yet) Write a Review
SKU:
7701-5
£135.37
Frequently bought together:

Description

Akt1, Active is available at Gentaur for Next week delivery.

Protein involved in cellular survival pathways

Biomolecule/Target:

Alternates names: Akt, RAC-alpha serine/threonine-protein kinase

Synonyms: Akt, RAC-alpha serine/threonine-protein kinase

Background Information: Akt1 or Protein Kinase B α(PKBα) is a serine/threonine kinase that belongs to the Akt family. Akt1 is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, and cell motility (1). The PI3K generates phosphatidylinositol-3,4,5-trisphosphate (PIP3), a lipid second messenger essential for the translocation of Akt1 to the plasma membrane where it is phosphorylated and activated by phosphoinositide-dependent kinase-1 (PDK-1) (2) and phosphoinositide-dependent kinase-2 (possibly ILK) (3). Akt1 has numerous cellular substrates including proteins, which promote the inhibition of apoptosis such as the Forkhead transcription factors and the Bcl-2 family member Bad (4). In addition, the cyclin dependent kinase inhibitors are substrates of Akt which when phosphorylated relinquish their inhibitory influence on cell cycle progression. Akt also mediates many of the stimulatory effects of insulin on glucose metabolism through deactivation of glycogen synthase kinase, activation of phosphofructo-kinase, and modulation of glucose transporter activity. Akt1 has been shown to play a role in human cancers (4). It is frequently overexpressed and active in many types of human cancers including cancers of colon, breast, brain, pancreas and prostate as well as lymphomas and leukemias (5). Akt1 plays an important role in cancer cell survival and proliferation thereby contributing to cancer progression.

Reconstitution Instructions: N/A

NCBI Gene Symbol: AKT1

Gene ID: 207

NCBI Accession: P31749

View AllClose

Additional Information

Size:
5 μg
Country of Manufacturing Origin:
USA
Country of Animal Origin:
USA
Recombinant:
Yes
Source:
Baculovirus (Sf9 insect cells)
Purity by SDS-PAGE:
≥90%
Assay:
SDS-PAGE
Purity:
N/A
Assay 2:
HPLC
Endotoxin Level:
N/A
Activity (Specifications/test method):
124 nmol phosphate incorporated into Akt substrate peptide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP and total of 0.83 µCi/µl P-32.
Biological activity:
N/A
Results:
N/A
Molecular Weight:
~85.0 kDa
Storage Temperature:
-80°C
Shelf Life:
12 months
Concentration:
5 µg/50 µl
Appearance:
Liquid
View AllClose

0 Reviews

View AllClose