BioVision Products
Active Sonic Hedgehog (SHH), murine recombinant
- SKU:
- 4020-100
Description
Active Sonic Hedgehog (SHH), murine recombinant is available at Gentaur for Next week delivery.
Hedgehog signaling pathway ligand involved in regulating vertebrate organogenesis
Biomolecule/Target: Sonic Hedgehog (SHH)
Alternates names: SHH; HHG1; HLP3; HPE3; SMMCI, Sonic hedgehog
Synonyms: SHH; HHG1; HLP3; HPE3; SMMCI, Sonic hedgehog
Background Information: Recombinant Mouse Sonic Hedgehog is a member of a small group of secreted proteins that are essential for development in both vertebrates and invertebrates. Three mammalian hedgehog genes (sonic, desert, Indian) share about 60% homology. The Mouse Sonic Hedgehog is 99% homologous to the human gene. Cysteine at position 25 has been substituted with Ile. Mouse SHH is a non-glycosylated polypeptide consisting of 176 amino acids with a molecular weight of 19,800 Da.
Reconstitution Instructions: When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile H₂O to a concentration of 0.1-0.5 mg/ml, which can be further diluted into other aqueous solutions.
NCBI Gene Symbol: SHH
Gene ID: 20423
NCBI Accession: Q8C765
Additional Information
Size: |
100 μg |
Adwords: |
Yes |
Country of Manufacturing Origin: |
USA |
Country of Animal Origin: |
USA |
Gene Source: |
Mouse |
Recombinant: |
Yes |
Source: |
E. coli |
Purity by SDS-PAGE: |
≥97% |
Assay: |
SDS-PAGE |
Purity: |
≥97% |
Assay 2: |
HPLC |
Endotoxin Level: |
<0.01 ng/μg |
Activity (Specifications/test method): |
N/A |
Biological activity: |
Mouse SHH is fully biologically active when compared to standard. Determined by it’s ability to induce alkaline phosphatase production by C3H10T1/2 fibroblasts, with an expected ED₅₀ in the range of 0.03 – 0.30 ug/ml. |
Results: |
0.03 – 0.30 ug/ml. |
Molecular Weight: |
19.8 kDa |
Storage Temperature: |
-20°C |
Shelf Life: |
12 months |
Concentration: |
N/A |
Appearance: |
Lyophilized protein |
Handling: |
Centrifuge the vial prior to opening. |
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