Active Sonic Hedgehog (SHH), human recombinant

Active Sonic Hedgehog (SHH), human recombinant is available at Gentaur for Next week delivery.

Hedgehog signaling pathway ligand involved in regulating vertebrate organogenesis

Biomolecule/Target: Sonic Hedgehog (SHH)

Alternates names: SHH; HHG1; HLP3; HPE3; SMMCI, Sonic hedgehog

Synonyms: SHH; HHG1; HLP3; HPE3; SMMCI, Sonic hedgehog

Background Information: Recombinant Human Sonic Hedgehog (SHH) is a highly conserved protein that plays an important role in embryonic development. It is expressed in neural tissue, the gut, and areas of limb development and promotes differentiation and growth in a tissue-specific manner. SHH is synthesized as a 45-kDa precursor protein, which is then cleaved to generate the active 19-kDa N-terminus. SHH interacts with the Patched and Smoothened transmembrane receptors, leading to the activation of GLI family transcription factors. Disruption of any part of this pathway during embryogenesis is associated with birth defects ranging from mild to severe. In adults, abnormal activation of the SHH pathway has been implicated in several forms of cancer. Three mammalian hedgehog genes (sonic, desert, Indian) share about 60% homology. The Cys at position 25 has been substituted with Ile. Human SHH is a non-glycosylated polypeptide consisting of 175 amino acids with a molecular weight of 19,697 Da.

Reconstitution Instructions: When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile H₂O to a concentration of 0.1-0.5 mg/ml, which can be further diluted into other aqueous solutions.

NCBI Gene Symbol: SHH

Gene ID: 6469

NCBI Accession: Q15465