Rabbit anti-EBOV VP30 pAb

Rabbit anti-EBOV VP30 pAb is available at Gentaur for Next Week Delivery.

Product Type: polyclonal antibody

Host: Rabbit

Clonality: polyclonal

Applications: ELISA, WB

Immunogen: Peptide sequence specific to Ebola Virus (EBOV) VP30 phosphoprotein.

Description: Affinity purified rabbit polyclonal antibody reactive to EBOV VP30. The antibody detects EBOV VP30 in
irradiated virus in Western Blot and ELISA.

Supplied: 100 µg is supplied in PBS + 0.02% sodium azide at a concentration of 0.575 mg/mL.

Purification: Antibody is affinity purified using immobilized immunogen.

Relevance: The antibody can be used for detection of a distinct epitope near the N-terminus of EBOV VP30 protein.

Recommended Dilutions:
ELISA: Assay-dependent dilution.
WB: Assay-dependent dilution.

Storage: 2-3 weeks +4°C, -80°C long term. Avoid multiple freeze thaws.

Cross Reactivity: Some nonspecific reactivity was observed against Sudan Virus (SUDV) or Marburg-Musoke
Virus (MARV) as demonstrated in the Western Blot.

Western Blot Data:

Anti-EBOV VP30 antibody at 500 ng/mL was able to detect
VP30 in heat-denatured and reduced irradiated EBOV virus
at 1:2 dilution (lane 1) by Western Blot Analysis. Some
nonspecific reactivity was observed with SUDV and MARV
irradiated virus (lanes 2 and 3, respectively). Western Blots
were developed using an anti-rabbit IgG-HRP conjugate and
TMB Membrane substrate.

ELISA Data:

Antigens were diluted to a 1:1000 dilution in PBS for plate
coating. Anti-EBOV VP30 antibody was serially diluted
semi-log from 10 µg/mL and incubated on the coated plates.
Washed plates were detected with anti-rabbit IgG-HRP
conjugate and TMB substrate. OD650 is reported above