Description
Rabbit anti-EBOV NP pAb is available at Gentaur for Next Week Delivery.
Product Type: polyclonal antibody
Host: Rabbit
Clonality: polyclonal
Applications: ELISA, WB
Immunogen: Peptide sequence specific to Ebola virus (EBOV) Nucleocapsid Protein (NP).
Description: Affinity purified rabbit polyclonal antibody reactive to EBOV NP. The antibody detects NP in virus-like particles (VLP) in Western blot and ELISA.
Supplied: 100 µg is supplied in PBS at a concentration of 0.37 mg/mL. 0.02% Sodium azide has been added.
Raised in: Rabbits
Purification: Antibody is affinity purified using immobilized immunogen.
Clonality: Polyclonal
Relevance: The antibody can be used for detection of EBOV NP.
Recommended Dilutions:
ELISA: Assay-dependent dilution
WB: Assay-dependent dilution. Internal QC demonstrates strong detection of EBOV NP in 500 ng of VLP at a use dilution of 100 ng/mL of the antibody.
Storage: 2-3 weeks +4◦C, -20◦C long term
Cross Reactivity: No cross-reactivity to NP in Sudan virus (SUDV) VLP or Marburg virus (MARV) VLP.
Related Products:
IBT provides a wide array of anti-filovirus specific antibodies,
recombinant proteins and other infectious disease reagents.
Please see our website, www.ibtbioservices.com for more details.
Western Blot Data:
Western blot was detected under reduced conditions with antiEBOV NP Pab at 100 ng/mL and visualized using an anti-rabbit IgG-HRP conjugate and TMB membrane substrate. NP is visualized in baculovirus-expressed EBOV VLP (lane 1) but not in
the SUDV or MARV VLPs (lanes 2, 3); nor in the EBOV VLP without NP expression (lane 4).
ELISA Data:
VLPs were solubilized in a final concentration of 1% Triton X-100
and diluted to 10 µg/mL in PBS (0.01% Triton) for plate coating.
Anti-EBOV NP antibody was serially diluted semi-log from 20
µg/mL and incubated on the coated plates. Washed plates were
detected with anti-rabbit IgG-HRP conjugate and TMB substrate.
OD650 is reported above.