Rabbit anti-EBOV GPDmuc pAb

Rabbit anti-EBOV GPDmuc pAb is available at Gentaur for Next Week Delivery.

Product Type: polyclonal antibody

Host: Rabbit

Clonality: polyclonal

Applications: ELISA, WB

Immunogen: Purified recombinant Ebola virus (EBOV) Glycoprotein (GP), modified.

Description: Purified rabbit polyclonal antibody reactive to EBOV GP. The antibody detects recombinant EBOV GP (modified) in Western blot and ELISA.

Supplied: 500 µg of antibody is supplied in DPBS at a concentration of 1.877 mg/mL.

Relevance: The antibody can be used for detection of EBOV GP.

Recommended Dilutions:

ELISA: Assay-dependent dilution.

WB: Assay-dependent dilution; internal QC demonstrates strong detection of 50 ng of purified recombinant EBOV GP (modified) with a use dilution from 2 µg per mL of antibody in diluent using an HRP antibody conjugate and chromogenic substrate for visualization.

Storage: 2-3 weeks +4°C, -80°C long term

Western Blot Data:

Western blots were detected with anti-EBOV GPΔmuc at 2 µg/mL
and visualized using an anti-rabbit HRP conjugate and
chromogenic substrate. Heat-denatured and reduced GP is
visualized as multiple bands representing differing glycosylation
patterns and GP subunits as observed above: EBOV Makona
rGPΔTM (lane 1) and EBOV Mayinga rGPΔmuc (lane 2)

rGPΔTM and rGPΔmuc proteins were diluted to 1 µg/mL for plate
coating. Anti-EBOV GPΔmuc polyclonal antibody was serially
diluted semi-log from 10 µg/mL and incubated on the coated
plates. Washed plates were detected with anti-rabbit IgG-HRP
conjugate and TMB substrate. OD650 is reported above.