Mouse anti-EBOV GP mAb (4F3)

Mouse anti-EBOV GP mAb (4F3) is available at Gentaur for Next Week Delivery.

Product Type: monoclonal antibody

Host: Mouse

Clonality: monoclonal

Applications: ELISA, WB

Immunogen: Purified recombinant Ebola Virus (EBOV) glycoprotein lacking the transmembrane region.

Description: Protein G purified mouse monoclonal antibody reactive to EBOV glycoprotein (GP). The antibody detects glycoprotein in virus-like particles (VLP) and EBOV glycoprotein without the transmembrane region (rGPΔTM).

Supplied: 100 µg is supplied in PBS at a concentration of 1.36 mg/mL. No preservative is added.

Clonality: Monoclonal of the IgG2a isotype

Relevance: The antibody can be used for detection of EBOV GP.

Recommended Dilutions:
ELISA: Assay-dependent dilution
WB: Assay-dependent dilution. Internal QC demonstrates good detection when using 4F3 mAb at a use dilution of 0.5 µg/mL.

Storage: 2-3 weeks +4oC, -80◦C long term

Cross Reactivity: No cross-reactivity was observed to Sudan Virus (SUDV) or Marburg Virus (MARV) rGPΔTM protein and VLP.

Western Blot Data:

Western blots were detected under reduced conditions with antiEBOV GP 4F3 at 0.5 µg/mL and visualized using an anti-mouse IgGHRP conjugate and TMB Membrane substrate. GP is visualized in
EBOV rGPΔTM (lane 1) and in Baculovirus-expressed EBOV VLP
(lane 4). No cross-reactivity with SUDV or MARV GP was observed.

ELISA Data:

rGPΔTM proteins and VLPs were diluted to 1 µg/mL and 10
µg/mL, respectively, in PBS for plate coating. Anti-EBOV GP
antibody 4F3 was serially diluted semi-log from 10 µg/mL and
incubated on the coated plates. Washed plates were detected with
anti-mouse IgG-HRP conjugate and TMB substrate. OD650 is
reported above.