Biomatik Elisa
Human PIAS1 (Protein Inhibitor of Activated STAT 1) ELISA Kit
- SKU:
- EKE60601
- Availability:
- Next Week Delivery
Description
Human PIAS1 (Protein Inhibitor of Activated STAT 1) ELISA Kit is available at Gentaur for next week delivery.
Specificity:
Cross Activity: No significant cross-reactivity or interference was observed.
Test principle: This ELISA Kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PIAS1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PIAS1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PIAS1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PIAS1. The concentration of Human PIAS1 in samples can be calculated by comparing the OD of the samples to the standard curve.
Assay procedure summary:
Stability:
Restrictions: For Research Use Only. Not for use in diagnostic procedures, drug use, or for administration to humans or animals.
Quality Systems: The kit is manufactured at ISO 9001 certified facilities.
Quality Assurance:
Research Area: Cancer, Epigenetics and Nuclear Signaling
Target Synonyms: PIAS1
Precision:
Additional Information
Size: |
96-Well Plate |
Species Reactivity: |
Human |
Sample type: |
Serum, Plasma, Biological Fluids |
Detection range: |
62.5-4000 pg/mL |
Sensitivity: |
37.5 pg/mL |
Detection Method: |
Colorimetric |
Assay Time: |
4.5 hours |
Application: |
ELISA |
Assay Type: |
Sandwich |
Shipping Condition: |
Ice packs |
Storage: |
Short term: 4°C; Long term: see manual. |
Precaution of Use: |
The Stop Solution is acidic. Do not allow to contact skin or eyes. |
Expiry Date: |
6 months |
UniProt ID: |
O75925 |