Human CD4 (Cluster of Differentiation 4) ELISA Kit

(No reviews yet) Write a Review
SKU:
EKE60495
Availability:
Next Week Delivery
£482.20
Frequently bought together:

Description

Human CD4 (Cluster of Differentiation 4) ELISA Kit is available at Gentaur for next week delivery.

Specificity:

Cross Activity: No significant cross-reactivity or interference was observed.

Test principle: This ELISA Kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CD4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CD4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CD4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CD4. The concentration of Human CD4 in samples can be calculated by comparing the OD of the samples to the standard curve.

Assay procedure summary:

Stability:

Restrictions: For Research Use Only. Not for use in diagnostic procedures, drug use, or for administration to humans or animals.

Quality Systems: The kit is manufactured at ISO 9001 certified facilities.

Quality Assurance:

Research Area: Cancer, Immunology, Stem cells

Target Synonyms: CD4

Precision:

 

View AllClose

Additional Information

Size:
96-Well Plate
Species Reactivity:
Human
Sample type:
Serum, Plasma, Biological Fluids
Detection range:
0.31-20 ng/mL
Sensitivity:
0.19 ng/mL
Detection Method:
Colorimetric
Assay Time:
4.5 hours
Application:
ELISA
Assay Type:
Sandwich
Shipping Condition:
Ice packs
Storage:
Short term: 4°C; Long term: see manual.
Precaution of Use:
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Expiry Date:
6 months
UniProt ID:
P01730
View AllClose

0 Reviews

View AllClose