Chicken Amylase, Alpha 2A (AMY2A) ELISA Kit

€808.00
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SKU:
EKU10632
Availability:
Next Week Delivery
Size:
96-Well Plate
Target Name:
Amylase, Alpha 2A
Species Reactivity:
Chicken (Gallus)
Sample type:
Serum, plasma, tissue homogenates and other biological fluids
Detection range:
15.6-1,000 pg/mL
Sensitivity:
6.1 pg/mL
Detection Method:
Colorimetric
Assay Time:
3 hours
Application:
ELISA
Assay Type:
Double-antibody Sandwich
Shipping Condition:
Ice packs
Storage:
Short term: 4°C; Long term: see manual.
Precaution of Use:
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Expiry Date:
8 months
UniProt ID:
n/a
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Chicken Amylase, Alpha 2A (AMY2A) ELISA Kit is available at Gentaur for next week delivery.

Specificity: This assay has high sensitivity and excellent specificity for detection of Amylase, Alpha 2A (AMY2A).

Cross Activity: This assay has high sensitivity and excellent specificity for detection of Amylase, Alpha 2A (AMY2A).

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amylase, Alpha 2A (AMY2A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Amylase, Alpha 2A (AMY2A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Amylase, Alpha 2A (AMY2A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Amylase, Alpha 2A (AMY2A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100 µL standard or sample to each well. Incubate 2 hours at 37°C; 3. Aspirate and add 100 µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100 µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90 µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50 µL Stop Solution. Read at 450nm immediately.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Restrictions: For Research Use Only. Not for use in diagnostic procedures, drug use, or for administration to humans or animals.

Quality Systems: The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.

Quality Assurance: Biomatik ELISA Kits are not typically pre-assembled as finished products due to limited shelf life. Before shipping, each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Detection Range, Sensitivity, and Precision. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. Please always refer to the hard copy manual included in The kitbefore experiment.

Research Area: Enzyme & Kinase;Metabolic pathway;Infection immunity;Hepatology;

Target Synonyms: AMY2; AMY2B; PA; 1,4-alpha-D-glucan glucanohydrolase

Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Amylase, Alpha 2A (AMY2A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Amylase, Alpha 2A (AMY2A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Intra-assay: CV<10%. Inter-Assay: CV<12%.