Tyrosinase Activity Assay Kit (Colorimetric)

Tyrosinase Activity Assay Kit (Colorimetric)is available at Gentaur for Next week Delivery.

The first non-radioactive kit on the market that measure Tyrosinase activity in biological samples.

Description:

Tyrosinase (EC 1.14.18.1) is a copper-binding enzyme that is expressed across a vast range of species ranging from bacteria and fungi to mammals. It is involved in two sequential reactions of the melanin synthesis pathway; first being the hydroxylation of a monophenol and second the conversion of an ortho-diphenol to a quinone. Quinone then undergoes a series of reactions including polymerization to form melanin. Tyrosinase is of great interest to the agriculture industry since it causes browning of fruits, vegetable and mushrooms, as well as to the cosmetic industry as it causes skin darkening. Development and screening of tyrosinase inhibitors, therefore is very useful for conditions such as hyperpigmentation and melasma. Tyrosinase activity is significantly increased in melanoma. Therefore, the detection of tyrosinase activity could be promising as a specific diagnostic test for melanoma and may be useful in monitoring patient response to melanoma treatments. BioVision’s Tyrosinase Activity Assay Kit is a simple one-step, plate-based assay for the measurement of tyrosinase activity in biological samples. In this assay, tyrosinase catalyzes the conversion of a phenolic substrate to a Quinone intermediate, which reacts with the tyrosine enhancer forming a highly stable chromophore with absorbance at 510 nm. The assay can detect as low as 30 µU Tyrosinase in biological samples

Applications: • Measurement of Tyrosinase Activity in various tissue/cell lysates
• Analysis of Tyrosinase activity in food chemistry/skin-related research

Sample Typ:e • Animal tissues: liver, heart, kidney, etc.
• Cell culture: Adherent and suspension cells (e.g melanoma cells)
• Plant tissues: potato, etc.
• Purified/recombinant protein/preparations

Alternate Name:

Features and Benefits:

• First non-radioactive assay in the market
• Simple, rapid & convenient
• The assay can detect Tyrosinase Activity less than 30 µU in biological samples.