Triose Phosphate Isomerase (TPI) Activity Colorimetric Assay Kit

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SKU:
K670-100
€545
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Description

Triose Phosphate Isomerase (TPI) Activity Colorimetric Assay Kitis available at Gentaur for Next week Delivery.

Only Kit on the market to measure Triose Phosphate Isomerase (TPI) Activity.

Description:

Triose Phosphate Isomerase (TPI or TIM: EC 5.3.1.1) is an important enzyme for glycolysis. It reversibly interconverts dihydroxyacetone phosphate and glyceraldehyde-3-phosphate, thus maintaining the equilibrium of these two triose phosphates. TPI connects glycolysis to pentose phosphate pathway and lipid metabolism. It is a stable homodimer found in almost all organisms. In humans, TPI deficiency is a rare multisystem disorder and leads to progressive neurological dysfunction, characterized by hemolytic anemia, cardiomyopathy and progressive neuromuscular impairment. BioVision’s Triose Phosphate Isomerase Activity Assay kit provides a quick and easy way for monitoring Triose Phosphate Isomerase activity in a variety of samples. In this kit, Triose Phosphate Isomerase converts dihydroxyacetone phosphate into glyceradehyde-3-phosphate, which reacts with the Enzyme Mix & Developer to form a colored product with strong absorbance at 450 nm. The assay is simple, sensitive, & high-throughput and can detect Triose Phosphate Isomerase activity as low as 40 mU/ml.

Applications: • Measurement of TPI activity in various tissues and cells • Analysis of glycolysis & pentose phosphate pathway and lipid metabolism

Sample Typ:e • Animal tissues: muscle, liver, heart, kidney, etc. • Cell culture: Adherent or suspension Cells • Human serum or plasma

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Features and Benefits:

• Simple, highly sensitive, and high-throughput. • The assay can measure Triose Phosphate Isomerase activity as low as 40 mU/ml.

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Additional Information

Size:
100 assays
Storage Conditions:
4°C-20°C
Shipping Conditions:
gel pack
Shelf life:
12 months
Detection Method:
Absorbance (OD 450 nm)
Category:
Triose Phosphate Isomerase Activity
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