Phosphatidylcholine Colorimetric/Fluorometric Assay Kit

Phosphatidylcholine Colorimetric/Fluorometric Assay Kitis available at Gentaur for Next week Delivery.

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Description:

Phosphatidylcholine (PC) is a phospholipid which incorporates choline as the headgroup of the lipid. PC is a major constituent of biological membranes and is involved in cell signaling through release of choline by phospholipase D leaving the second messenger phosphatidic acid. BioVision's Phosphatidylcholine Assay Kit is a simple convenient means of measuring Phosphatidylcholine in a variety of biological samples. The kit utilizes an enzyme-coupled assay in which PC is hydrolyzed, releasing choline which is subsequently oxidized resulting in development of the OxiRed probe to generate fluorescence (Ex/Em 535 nm 587 nm) and absorbance (570 nm). BioVision’s Phosphatidylcholine kit measures PC in the range of 0.1 to 10 nmol per sample. PC is present in serum at ~ 0.2-2.5 mM (~50-200 mg/dL).

Applications:

Sample Typ:e Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluids

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Features and Benefits:

• Simple procedure; takes less than ~40 minutes • Fast and convenient