Neprilysin (Human) ELISA Kit

Neprilysin (Human) ELISA Kitis available at Gentaur for Next week Delivery.

A Sandwich ELISA kit for the quantitative measurement of Neprilysin in human serum, plasma, tissue lysates, and other biological fluids.

Description:

Neprilysin (MME, CD10) is a zinc-dependent membrane metallopeptidase located on the surface of neutrophils, endothelial cells, and a variety of tissues such as brain, heart, kidneys, and lungs. It catalyzes the degradation of peptides such as atrial (ANP), B-type (BNP), and C-type (CNP) natriuretic peptides, Angiotensin-II, Bradykinin, and Endothelin-1. It also degrades amyloid-beta peptide in the brain and slows the progression of Alzheimer’s disease. Additionally, it is an important cell surface marker in the diagnosis of hematologic diseases such as human acute lymphocytic leukemia (ALL), Burkitt lymphoma, and diffuse large B-cell lymphoma. BioVision’s Neprilysin (Human) ELISA kit is based on the Sandwich ELISA principle to quantitatively measure the amount of Neprilysin in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and subsequently washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed away with Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of Neprilysin in the sample or standard.

Applications: This Sandwich ELISA kit is designed to quantitatively measure amount of Neprilysin in human serum, plasma, tissue lysates and other biological fluids

Sample Typ:e Plasma,Serum,Tissue lysate,Other biological fluids

Alternate Name: NEP, SFE, CD10, CALLA, CMT2T, SCA43

Features and Benefits:

Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%,Recovery range: 85 - 105% for normal human serum and plasma samples,This Sandwich ELISA is highly sensitive and highly specific for the detection of Neprilysin in human samples. There is no significant cross-reactivity or interference between Neprilysin and analogues,Sensitivity: 0.094 ng/ml,Detection range: 0.156 - 10 ng/ml