Lipoprotein Lipase Activity Fluorometric Assay Kit

Lipoprotein Lipase Activity Fluorometric Assay Kitis available at Gentaur for Next week Delivery.

Highly Sensitive Assay, HTS.

Description:

Lipoprotein lipase (LPL) is a member of the lipase family that hydrolyzes triglycerides in chylomicrons and very low-density lipoprotein (VLDL). Digestion of triglycerides in VLDL by LPL leads to their conversion to intermediate-density lipoprotein (IDL) and then low-density lipoprotein (LDL). LPL is found attached to the luminal surface of endothelial cells in the heart, muscle, and adipose tissue. Mutations in lipoprotein lipase can lead to a variety of disorders such as lipoprotein metabolism, hypertriglyceridemia etc. Overexpression of LPL in mice has been shown to promote obesity and insulin resistance. BioVision’s LPL Activity Assay Kit contains a quenched substrate that fluoresces upon hydrolysis by LPL. The fluorometric intensity is directly proportional to the amount of substrate hydrolyzed. This assay detects total lipase activity when no inhibitor is used. Comparing results in the presence or absence of an LPL inhibitor allows for quantification of LPL activity specifically. Our results indicate that the majority (~90%) of lipase activity detected by this kit in post-heparin treated mouse plasma is from LPL. To determine the exact LPL specific activity in mouse plasma, measure activity in pre- and post-heparin treated plasma.

Applications: • Measurement of LPL activity in purified wild type or recombinant protein as well as in plasma, cell and tissue lysates

Sample Typ:e • Pure (wild type or recombinant) LPL protein • Plasma • Cell lysate and tissue (to determine LPL activity specifically compare results in the presence and absence of LPL inhibitor)

Alternate Name:

Features and Benefits:

• Simple procedure, wide applicability • Fast and convenient • Highly Sensitive Fluorometric assay for measuring Lipoprotein Lipase activity in various biological samples