Leucine Aminopeptidase (LAP) Activity Assay Kit (Fluorometric)

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SKU:
K534-100
€445
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Description

Leucine Aminopeptidase (LAP) Activity Assay Kit (Fluorometric)is available at Gentaur for Next week Delivery.

Highly Sensitive, minimum sample preparation required.

Description:

Leucine aminopeptidases (EC 3.4.11.1) (LAPs) are a diverse set of exopeptidases that catalyze the hydrolysis of leucine residues from the amino-termini of proteins or peptides. LAPs are ubiquitous enzymes present among animals, plants and prokaryotes. Previously, they were thought to typically play important roles in cell maintenance, growth and development. However, research in the recent years has identified multiple secondary functions for these enzymes in animals and microbes including transcriptional regulation and vesicle transport. Studies have implicated LAP enzymes in tumor cell proliferation, invasion and angiogenesis. Placental LAP is used as a biomarker in ovarian epithelial cancer while adipocyte-derived LAP is used as a marker of endometrial cancer cell proliferation and differentiation. LAP enzymes are also known to be involved in catabolism of oxytocin and vasopressin and insulin regulation of GLUT4 receptors in diabetes. BioVision’s Leucine Aminopeptidase assay kit provides a quick, sensitive and easy way for measuring total LAP activity in various samples. In this assay, LAPs hydrolyze leucine from the fluorescent probe and the amount of fluorescent probe detected at Ex/Em 368/460 nm is used to determine the total activity of the LAP enzymes. The assay is simple to perform, high-throughput adaptable and can detect less than 0.1 mU of LAP activity

Applications: Detection of LAP activity in mammalian tissues, cell culture and purified enzyme

Sample Typ:e • Mammalian tissues: liver, spleen, placenta • Cell culture: adherent or suspension cells

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Features and Benefits:

• Simple one-step reaction • Takes only 1-2 hrs • Non-radiometric fluorescent detection • HTP adaptable

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Additional Information

Size:
100 assays
Storage Conditions:
4°C-20°C
Shipping Conditions:
gel pack
Shelf life:
12 months
Detection Method:
Fluorometric (Ex/Em = 368/460 nm)
Category:
Protease Activity
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