Isopropanol Dehydrogenase Activity Assay Kit (Colorimetric)

Isopropanol Dehydrogenase Activity Assay Kit (Colorimetric)is available at Gentaur for Next week Delivery.

Simple and sensitive colorimetric assay for NADP dependant Isopropanol Dehydrogenase (ADH1-NADP) Activity in varied sample types..

Description:

NADP-dependent Isopropanol Dehydrogenase (ADH1-NADP, EC:1.1.1.80) belongs to the superfamily of alcohol dehydrogenases (Alcohol DHs, ADHs, EC 1.1.1.1) with a preference for medium chain secondary alcohols, such as 2-butanol and isopropanol. Isopropanol dehydrogenase facilitates the conversion between secondary alcohols to aldehydes and ketones with the reduction of NADP+ to NADPH. BioVision’s Isopropanol Dehydrogenase Activity Colorimetric Assay Kit provides a convenient tool for sensitive detection of the ADH1-NADP in a variety of samples. In this assay, Isopropanol gets converted to Acetone and NADPH in the presence of NADP+. This results in the development of colour in the presence of the developer. This colour is proportional to the ADH1-NADP levels in the sample and can be measured at λ = 450 nm. This assay detects ADH1-NADP activity as low as 0.001 mU in samples.

Applications: • Measurement of Isopropanol Dehydrogenase (ADH1-NADP) activity in various cells. • Measurement of NADP-dependent alcohol dehydrogenases’ activity with broad substrate specificity in various tissues/cells.

Sample Typ:e • Bacterial, yeast or protozoan samples: Entamoeba histolytica, Clostridium beijerinckii, Mycoplasma pneumonia, etc. • Animal tissues: such as liver, pancreas, small intestine, etc. • Animal cells.

Alternate Name: Alcohol Dehydrogenase (NADP dependant) Activity Assay Kit (Colorimetric)

Features and Benefits:

Simple, colorimetric protocol. Detects ADH1-NADP activity as low as 0.001 mU in samples. Compatible with multiple sample types.