HDAC3 Immunoprecipitation (IP) & Activity Assay Kit

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SKU:
K344-25
€645
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Description

HDAC3 Immunoprecipitation (IP) & Activity Assay Kitis available at Gentaur for Next week Delivery.

Only kit on the market for both IP and measuring activity of HDAC3.

Description:

Histone deacetylases (HDACs) play a central role in controlling cell cycle regulation, cell differentiation, and tissue development. These proteins have crucial roles in development and physiology, especially in the heart and nervous system. They are also deeply involved in cellular proliferation, cell cycle and apoptosis. HDAC3 is primarily localized in the nucleus, but can also be found in the cytoplasm and at the plasma membrane. The HDAC3 IP & Activity Assay Kit provides an antibody-based method to specifically immunoprecipitate the HDAC3 complex from cells & tissues and to measure HDAC3 activity fluorometrically. HDA3 is immunoprecipitated from cell or nuclear extract(s) using HDAC3 antibody followed by capturing the complex on protein-A/G beads. The immunoprecipitated complex is incubated with the HDAC substrate. Only the deacetylated substrate is cleaved by the Developer to produce a fluorophore, which can be easily analyzed using a fluorescence plate reader.

Applications:

Sample Typ:e Human, Mouse or Rat • Cell lysate and Nuclear Extract

Alternate Name: Histone Deacetylase 3 Immunoprecipitation (IP) & Activity Assay Kit

Features and Benefits:

• Simple, fast and convenient. • Only kit on the market for both IP as well as measuring activity of HDAC3.

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Additional Information

Size:
25 assays
Storage Conditions:
4°C-20°C
Shipping Conditions:
gel pack
Shelf life:
12 months
Category:
HDAC
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