HAT Activity Colorimetric Assay Kit

HAT Activity Colorimetric Assay Kitis available at Gentaur for Next week Delivery.

Non-radioactive assay for end point & kinetic studies.

Description:

Histone acetyltransferases (HATs) have been implicated to play a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation. BioVision’s HAT Activity Colorimetric Assay Kit offers a convenient, nonradioactive system for a rapid and sensitive detection of HAT activity in mammalian samples. The kit utilizes active Nuclear Extract (NE) as a positive control and acetyl-CoA as a cofactor. Acetylation of peptide substrate by active HAT releases the free form of CoA which then serves as an essential coenzyme for producing NADH. NADH can easily be detected spectrophotometrically upon reacting with a soluble tetrazolium dye. The detection can be continuous and suitable for kinetic studies. The kit provides a simple, straightforward protocol for a complete assay.

Applications:

Sample Typ:e Cell and tissue lysates

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Features and Benefits:

• Simple procedure • Fast and convenient • The detection can be continuous and suitable for kinetic studies