Glucose Uptake Fluorometric Assay Kit

Glucose Uptake Fluorometric Assay Kitis available at Gentaur for Next week Delivery.

Non-radioactive, Sensitive Assay.

Description:

Glucose uptake is an important biological process for studying cell signaling and glucose metabolism. Among many different methods available for measuring glucose uptake, 2-deoxyglucose (2-DG) has been widely used because of its structural similarity to glucose. As with glucose, 2-DG can be taken up by glucose transporters, and metabolized to 2-DG-6-phosphate (2-DG6P). 2-DG6P, however, cannot be further metabolized, and thus accumulates in the cells. The accumulated 2-DG6P is, therefore, directly proportional to 2-DG (or glucose) uptake by cells. In BioVision’s glucose uptake fluorometric assay kit, the accumulated 2-DG6P is enzymatically oxidized and coupled to BioVision’s PicoProbe, which generates fluorescence in the presence of NADPH. This easy to use non-radioactive kit can detect glucose uptake as low as 50 pmol/well and can be used for a variety of cell types.

Applications:

Sample Typ:e Cell lysates

Alternate Name:

Features and Benefits:

• Simple procedure • Fast and convenient • The assay is easy, non-radioactive, and highly sensitive